摘要
150只SD大白鼠随机分成5组，雌雄各半，对照组饮饲蒸馏水，试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组分别添加钒10mg／kg、20mg／kg、40mg／kg、60mg／kg，于饮饲后第2、4、6、8、10周断脊处死，取脾脏称重并制作石蜡切片，HE染色，显微观察，研究微量元素钒对大白鼠脾脏生长发育的影响。结果表明，饮饲10mg／kg、20mg／kg、40mg／kg钒，可见大白鼠脾脏重量较同一周龄对照组都有不同程度的增加，其中以20mg／kg组脾脏增重极显著（P〈0．01）；显微观察可见10mg／kg组、20mg／kg组、40mg／kg组脾小结数量增多变大，动脉周围淋巴鞘增厚，椭球管腔变大，以10mg／kg组，20mg／kg组最为显著。60mg／kg组脾脏重量与同一周其他剂量组相比较轻，脾小结数量少且动脉周围淋巴鞘薄。说明大白鼠饮水中添加10～40mg／kg钒是安全的，其中20mg／kg钒对脾发育有促进作用，60mg／kg钒对大白鼠脾具有抑制作用。
The 150 SD rats were divided into five groups,the control group drinks distilled water, the experiment Ⅰ , Ⅱ ,Ⅲ and IV groups were appended by the Vanadium dosage of 10 mg/kg, 20 mg/kg, 40 mg/kg and 60mg/kg, after 2, 4, 6, 8 and 10 weeks, executed with break ridge, with the spleen taken and weighted, doing Paraffin slice, made use of optical microscopy to observe organizational structure, to study the effect of fraeeelement Vanadium on spleen grnwth in SD rat. Results showed that when the Vanadium dosage is 10mg/kg,20mg/ kg,40 mg/kg, the weight is to increase with comparison, the Exp Ⅱ is obvious, Microscopic observation Visibility,the Quantity of Splenic nodule increase, the Periarterial lymphatic sheath is thickened, the tube of Oval ball largened, the Exp Ⅰ and Exp Ⅱ is obviously. The weight of ExpⅣ is light, the quantity of splenic nodule is reduced, the periarterial lymphatic sheath is wafery. It showed that when the Vanadium dosage is under 40 mg/ kg, it is safe to the white rat. It has the stimulative action to the spleen growth when the dosage is 20 mg/kg, when the dosage is 60mg/kg, it has inhabit effect.
出处
《安徽科技学院学报》
2008年第3期4-8,共5页
Journal of Anhui Science and Technology University
基金
安徽科技学院引进人才基金(ZRC200678)
