摘要
目的探索大鼠脑干中缝核群星形胶质细胞对睡眠剥夺(SD)的反应及其与神经元的关系。方法采用小平台水环境法建立大鼠SD模型,分为睡眠剥夺12h(SD)组,睡眠剥夺12h恢复睡眠3h(SR)组及大平台对照(TC)组和正常单独饲养(CC)组,每组3只。用免疫组化的方法观察c-fos和胶原纤维酸性蛋白(GFAP)在脑干中缝核群的表达,及其与5-羟色胺(5-HT)阳性神经元的关系。结果GFAP在中缝核群尤其是中缝背核表达明显增多,睡眠恢复后表达明显减少,其变化趋势及部位与c-fos基因表达相一致。在中缝背核形成3种形式的复合体样结构:(1)c-fos(+)、5-HT(+)、GFAP(+);(2)5-HT(+)、GFAP(+)、c-fos(-);(3)5-HT(-)、GFAP(+)、c-fos(+)。结论GFAP对睡眠剥夺和恢复睡眠的影响反应敏感,与神经元反应趋势一致,提示星形胶质细胞和神经元可能共同参与睡眠调节。
Objective To investigate the response of astrocyte in raphe nucleus to sleep deprivation, as well as its relationship with neurons. Methods Two group of Sprague Dawley rats were sleep deprived by housing them on the small platforms over water for 12h. After that one group can sleep 3h. Control group were housed in the normal cageor on the large platform over water. The expression of c-fos,GFAP and 5-HT positive reaction in the raphe nucleus were studied with triple method combining anti GFAP, c-fos and 5-HT immunocytochemistry staining. Results Sleep deprivation resulted in an increasing expression of c-fos and GFAP positive staining in the raphe nucleus. The main field were dorsal raphe nucleus. After sleep recovery, the expression of c-fos and GFAP positive staining decreased significantly. The change of GFAP positive astrocyte is similar to c-los positive neurons. Many of c-fos and 5-HT single labeled neurons,or c-fos and 5-HT double labeled neurons in the dorsal raphe nucleus surrounded by GFAP positive processes were found and formed three kinds of complex-like structures. Conclusion It＇ s indicated that astrocyte is sensitive to sleep deprivation and sleep recovery. It suggested that astrocytes and neurons might be involved in the regulation of sleep together.
出处
《疑难病杂志》
CAS
2008年第6期344-346,F0003,共4页
Chinese Journal of Difficult and Complicated Cases
