摘要
根据神经胶质细胞中星形胶质细胞、少突胶质细胞的生长存在时间差异，细胞生长方式及细胞对培养层粘附等特性不同，作者建立了体外分离培养方法。经光镜、扫描电镜证实，分离培养的细胞形态与先前用其它方法的研究结果相一致。免疫细胞化学鉴定显示星形胶质细胞的标志蛋白胶原纤维酸性蛋白（ Ｇ Ｆ Ａ Ｐ）和少突胶质细胞标志蛋白髓鞘碱性蛋白（ Ｍ Ｂ Ｐ）的表达与分离的细胞相符。表明所分离细胞的纯度较好，该方法可靠易行。
Based on the different properties of cellular adhesions,developmental time\|courses and growth pattern of astroglial cells and oligodendrocytes,a method of isolating glial cells in vitro was developed.The observations under inverted light microscope and scan electronic microscope identified that the morphology of isolated cells were consistent with the previous description.Immumocytochemical examination showed the expressions of markers,the gilal fibrillary acidic protein(GFAP) of astroglia cells and the myelin basic protein(MBP) of oligodendrocytes were consistent with the isolated cells.All parameters showed that the isolated glial cells were pure,and the new methods were easy and reliable.
出处
《卫生研究》
CAS
CSCD
北大核心
1999年第5期263-266,共4页
Journal of Hygiene Research
基金
四川省计生委资助
