摘要
应用序列特异性寡核苷酸探针反向斑点杂交技术对内蒙古地区蒙古族106名无关健康个体的HLA-A、B和DRB1基因座进行基因分型,以研究内蒙古地区蒙古族人群HLA-A、B、DRB1基因座的等位基因及其组成的单倍型频率分布特征。采用最大数学预期值算法计算HLA基因座的等位基因频率和单倍型频率。106名内蒙古地区蒙古族个体的HLA-A、B、DRB1基因座分别检出13、29、13个等位基因。高频单倍型分别为HLA-A*02-B*46(0.0510);HLA-A*02-B*13(0.0495);HLA-A*02-B*51(0.0442);HLA-B*13-DRB1*07(0.0555);HLA-B*46-DRB1*09(0.0378);HLA-B*35-DRB1*13(0.03300);HLA-A*02-B*13-DRB1*07(0.033019);HLA-A*02-B*46-DRB1*09(0.031985)。研究表明:内蒙古地区蒙古族人群HLA基因座的等位基因和单倍型具有较高的遗传多态性。HLA-A*24-B*14,HLA-A*32-B*63在该民族具有极强的连锁不平衡。
We investigated the genetic polymorphisms of HI_A-A, B, DRB1 alleles and haplotype frequencies in Mongols from Inner Mongolia, China. A PCR-based reverse line-strip sequence specific oligonucleotide hybridization method was used to deter- mine the alleles of HI_A-A, B, DRB1 in 106 unrelated, healthy Mongol individuals of Inner Mongolia. AUelic and haplotype fre- quencies of these loci were calculated by the maximum likelihood estimator method. Altogether, 13 HLA-A alleles, 29 HLA-B alleles and 13 HLA-DRB1 alleles were observed in the population. The most frequent HLA A-B, B-DRB1, and A-B-DRB1 haplo- types were HLA-A＊O2-B＊46 （0.0510）, HLA-A＊O2-B＊13 （0.0495）, HLA-A＊O2-B＊51 （0.0442）; HLA-B＊13-DRBl＊07（O.0555）, HLA B＊46-DRBl＊09 （0.0378）, HLA-B＊35-DRBl＊13（O.033）; and HLA-A＊O2-B＊13- DRBl＊07（0.033019）, HLA-A＊O2-B＊46-DRBl＊09 （0.031985）, respectively. These results indicated that the HLA loci are highly polymorphic in Mongols of Inner Mongolia, and haplotypes HLA-A＊24-B＊14, HLA-A＊32-B＊63 have significant linkage disequih＇brium.
出处
《遗传》
CAS
CSCD
北大核心
2008年第2期164-168,共5页
Hereditas（Beijing)
基金
国家自然科学基金资助(批准号:30700470)~~
