摘要
目的:建立高效液相色谱法测定抑制血管紧张素转化酶活性的杭白菊黄酮类组分。方法:杭白菊75%乙醇提取物经石油醚、正丁醇萃取后得黄酮类组分为测试佯品。用反相高效液相色谱测定杭白菊黄酮类组分对血管紧张素转化酶的抑制活性,采用 Agilent Zorbax SB-C_(18)色谱柱(4.6 mm×150mm,5μm),流动相为0.08%磷酸溶液-乙腈,梯度洗脱10 min,流速1.5mL·min^(-1),检测波长228 nm,进样量20μL。结果:对照品马尿酸浓度存4～500 μmol·L^(-1)范围内线性良好(r=0.9999);低、中、高3种浓度的平均同收率分别为102.4%,97.1%,97.2%;低、中、高3种浓度的日内、日间 RSD 分别为1.5%,0.6%,3.3%和1.3%,2.5%,2.9%。结论:本方法操作简便、灵敏、准确,重复性好,适用于杭白菊黄酮类成分中血管紧张素转化酶抑制剂的筛选。
Objective ：To establish an HPLC method for the determination of flavonoids from Chrysanthemum which inhibited angiotensin converting enzyme. Method：The Chrysanthemum flavonoid as test samples were obtained using 75 % ethanol -extracts of Chrysanthemum and extracted successively by petroleum ether, n -butyl alcohol. The compounds inhibiting the activities of angiotensin converting enzyme from Chrysanthemum were determined by HPLC - UV. Agilent Zorbax SB - C18 chromatographic column（4.6 mm × 150 mm, 5 μm）was adopted with the gradient elution solvent system composed of 0.08% phosphoric acid solution -acetonitrile as the mobile phase. The flow rate was 1.5 mL · min^-1, the detection wavelength was set at 228 nm and the volume of injection was 20 μL. Results：The calibration curve was linear within the range of 4 to 500 μmol · L^-1（ r = 0. 9999 ）. The mean recoveries for three kinds of concentration of hippuric acid were 102.4% ,97.1% ,97.2% respectively. Intra - day and inter - day RSDs were 1.5% ,0.6% ,3.3% and 1.3% ,2.5% ,2.9% respectively. Conclusion：The method is simple, sensitive, accurate ,reproducible, and suitable for the determination of angiotensin converting enzyme inhibitory compounds of flavonoids in Chrysanthemum.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第5期674-677,共4页
Chinese Journal of Pharmaceutical Analysis
