摘要
目的研究新城疫病毒(NDV)合用中药黄芪在小鼠体内诱生内源性干扰素-α及对小鼠的免疫调节作用。方法实验分3部分,实验1:分别以NDV、黄芪及二者合用予小鼠口腔喷洒给药,连续10日后以ELISA法测定小鼠唾液中干扰素-α和SIgA的含量;实验2:分别以NDV、黄芪及二者合用予小鼠腹腔注射给药(黄芪灌胃方式给药),ELISA法测定给药10日后血清干扰素-α的含量;实验3:分为NDV、黄芪及二者合用的高、中、低三种剂量,给药方法同第二组,6天后MTT法测定脾脏NK细胞的活性。每组分别设置阴性对照。结果NDV和黄芪口腔给药能升高小鼠唾液干扰素-α(35.54±10.20IU/ml,5.69±1.35IU/ml)和SIgA含量(68.88±15.47ng/ml,44.32±9.87ng/ml),腹腔给药能诱生血清干扰素-α(120.54±29.21IU/ml,10.69±6.35IU/ml),并能激活NK细胞活性(50.4%,32.1%)。NDV合用黄芪组的诱生效果远高于单独使用组(98.55±16.95IU/ml,101.31±27.59ng/ml,262.55±26.91IU/ml,75.6%),显示出较强的协同作用。结论NDV合用黄芪能诱生小鼠内源性干扰素,并能发挥对小鼠SIgA和NK细胞的免疫调节作用。
Objective To study the induction of endogenous interferon-α（IFN-α） and immunological regulation of Newcastle Disease Virus（NDV）and Milkvetch Root（MR）combinating administrated in mice.Methods There were 3 parts.Part 1：the IFN-α and SIgA concentration in saliva were detected by ELISA after NDV and MR were sprinkled in mouse oral cavity for 10 days.Part 2：the level of IFN-α in serum were detected but the drug were intraperitoneal administrated.Part 3：the activity of NK cell in spleen were measured by MTT method after the same treatment as group 2 for 6 days.Results The concentration of IFN-α（35.54±10.20 IU/ml,5.69±1.35 IU/ml）and content of SIgA（68.88±15.47 ng/ml,44.32±9.87 ng/ml）in saliva had been promoted after NDV and MR administrated in oral cavity,and the serum level of IFN-α（120.54±29.21 IU/ml,10.69±6.35 IU/ml）had been induced and spleen NK cell（50.4%,32.1%）had been activated after it administrated in abdominal cavity.When NDV and MR combinating administrated,they synergistically enhanced the production of endogenous IFN-α and stimulated the immunological regulation, the detected data（98.55±16.95 IU/ml,101.31±27.59 ng/m,262.55±26.91 IU/ml,75.6%）were more higher than they were separated used（P〈0.05）.Conclusion NDV combinated with MR used in mice showed a strong IFN-α inducing capacity,and then it could contributed the immunoregulation action on sIgA and NK cell.
出处
《成都医学院学报》
CAS
2008年第1期24-26,60,共4页
Journal of Chengdu Medical College
