摘要
目的 探讨盐酸戊乙奎醚（PHC）对粘连性肠梗阻（AIO）大鼠肠黏膜免疫屏障功能的影响及可能机制.方法 将60只SD大鼠分为正常组（N组）、假手术组（S组）、生理盐水组（NS组）及盐酸戊乙奎醚组（P2、P4、P8组）.建立AIO模型.正常组不予处理,生理盐水组及假手术组均给予0.4 ml生理盐水,PHC组按0.2、0.4、0.8 mg/kg配成0.4 ml,于术后第7d开始连续肌注7d.ELISA法测量血清中IL-4、IL-10含量以及肠内容物中分泌型免疫球蛋白A（sIgA）含量.结果 与S组、NS组比较,P2、P4、P8组血清IL-4[（19.34±2.87）,（23.85±2.72）,（27.89±2.71） vs （6.18±1.42）,（8.37±2.69） ng/L）]、IL-10[（118.93±20.78）,（121.61±24.932）,（127.41±22.61） vs （78.19±19.41）,（42.72±21.31） ng/L]以及肠内容物中sIgA含量（0.64±0.089,0.99±0.093,1.02±0.12 vs 0.41±0.06,0.14±0.03 ng/L）均有明显增高,差异均有统计学意义（均P＜ 0.05）.与P2组比较,P4、P8组IL-4与sIgA水平升高更为明显,差异均有统计学意义（P＜ 0.05）.结论 中、高剂量PHC对AIO大鼠肠黏膜的免疫屏障有一定保护作用,且以高剂量更为显著,其机制可能与增加抗炎因子释放和抑制炎性因子产生有关.
Objective To investigate the effect and possible mechanism of penehyclidine hydrochloride （PHC） on the immune barrier of the intestinal mucosa in rats with adhesive intestinal obstruction （AIO）.Methods Sixty Sprague-Dawley （SD） rats were divided into the normal group （N group）,sham-operated group （S group）,normal saline group （NS group） and penehyclidine hydrochloride groups （P2,P4,and P8 groups）.AIO model was established.The N group did not receive any treatment,while the Group NS and Group S received intramuscular injection of 0.4 ml normal saline,and PHC groups received intramuscular injection of 0.4 ml PHC （dosing to 0.2,0.4 and 0.8 mg/kg for Groups P2,P4 and P8 groups,respectively） for 7 days starting from day 7 after the modeling procedure.ELISA was used to measure the serum levels of IL-4 and IL-10,and the level of secretory immunoglobulin A （sIgA） in intestinal contents.Results Compared with S and NS groups,P2,P4,and P8 groups had higher serum IL-4 [（19.34±2.87）,（23.85±2.72）,（27.89±2.71） vs （6.18±1.42）,（8.37±2.69） ng/L）],IL-10 [（118.93± 20.78）,（121.61 ±24.932）,（127.41 ±22.61） vs （78.19± 19.41）,（42.72±21.31） ng/L] and sIgA of intestinal contents As compared to P2 groups,P4 and P8 groups had higher.IL-4 and sIgA（All P ＜ 0.05）.Conclusion Moderate to high dosage of PHC can protect the immune barrier of the intestinal mucosa from injury after AIO in rats.Such protection may be related to the increased release of anti-inflammatory cytokines and suppressed production of inflammatory cytokines.
出处
《中华生物医学工程杂志》
CAS
2014年第3期212-214,共3页
Chinese Journal of Biomedical Engineering
基金
广州中医药大学创新基金（KAB110112K54）
关键词
肠梗阻
肠黏膜
免疫
Intestinal obstruction Intestinal mucosa Immunity
