摘要
目的:探讨RNA干扰Survivin基因对腺样囊性癌细胞生长的抑制效应。方法:设计、合成siRNA(smallinterfering RNA),构建真核表达载体pGenesil-shRNA-Survivin,将重组质粒导入人腺样囊性癌ACC-M细胞株。采用逆转录-聚合酶链反应(RT-PCR)、Western-blot法分别检测转染后的ACC-M细胞中Survivin mRNA和蛋白表达的变化,用噻唑蓝(MTT)法检测细胞的增殖,结果:测序证实真核表达载体构建成功,在pGenesil-shRNA-Survivin的ACC-M细胞株中,Survivin mRNA及蛋白表达明显降低,ACC-M细胞生长受到抑制。结论:pGenesil-shRNA-Survivin重组质粒能有效抑制腺样囊性癌细胞生长。
Objectine：To investigate inhibitory effect of growth on Adenoid cystic carcinoma cells lines by siRNA Survivin. Method： We designed and synthesized siRNA .Eukaryotic expression vector pGenesil-shRNA- Survivin was constructed .Recombinant Plasmid was transfected into ACC-M cells.The expression of Survivin mRNA and protein was detected by RT-PCR and Western-blot.The growth of Adenoid cystic carcinoma cells was detected by MTT. Result： The recombinant plasmid was successfully constructed .The expression of Survivin mRNA and protein was obviously reduced . ACC-M cells growth was inhibited. Conclusion： Recombinant plasmid pGenesil-shRNA-Survivin can specially suppress adenoid cystic carcinoma cells growth.
出处
《临床口腔医学杂志》
2008年第4期197-200,共4页
Journal of Clinical Stomatology
基金
重庆市自然科学基金资助(CSTC
2007BB5064)
