摘要
目的探讨高糖对体外培养小鼠前体成骨细胞MC3T3-E1活性的影响。方法将培养的MC3T3-E1细胞分为5.5 mmol/l正常糖对照组、12.5 mmol/I高糖组和25 mmol/L高糖组,观察不同浓度糖对MC3T3-E1细胞增殖、碱性磷酸酶(ALP)、矿化结节、骨钙素(OC)及Caspase-3的影响。结果与5.5 mmol/L正常糖对照组相比,12.5 mmol/L高糖组培养5 d细胞增殖和ALP分泌不受影响,在培养7 d和14 d时明显促进矿化结节形成,在培养5 d时促进细胞内OC表达,在培养3 d时促进Caspase-3的表达;而25mmol/I高糖组在培养5 d则可明显抑制细胞增殖及ALP分泌,在培养14 d减少矿化结节的形成,在培养第5 d时减少OC表达,在培养3 d时促进Caspase-3的表达。结论高糖可影响MC3T3-E1细胞活性,12.5 mmol/L糖在培养时间内不影响细胞增殖与分化,可促进矿化;25 mmol/L糖则对细胞产生明显的抑制效应。
Objective To investigate the effect of high glucose on the activity of MC3T3-E1 ceils. Methods MC3T3 E1 cells were divided into three groups： 5.5 mmol/L normal glucose control group, 12.5 mmol/L high glucose group and 25 mmol/L high glucose group. The effects of different concentrations of glucose on the MC3T3-E1 cells proliferation, ALP, mineralized nodules, osteoctastic cell （OC）, and caspase-3 were measured. Results Compared with 5.5 mmol/L normal glucose control group, 12. 5 mmol/L group had no effect on cell proliferation and ALP secretion （5d）, but it can promote cell mineralization（7d, 14d）. The secretion of OC （osteocalcin） was increased. However, the expression of caspase-3 was also increased. 25 mmol/L glucose group had a negative impact after 5 days treatment and the secretion of caspase-3 was also promoted. Conclusion High glucose can affect the activity of MC3T3-E1 cell. 12.5 mmol/L glucose had no effect on cell differentiation and proliferation while it can promote cell mineralization. However 25 mmol/L glucose may have an inhibition effect during long-term treatment.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2011年第2期152-154,共3页
Chinese Journal of Diabetes
