摘要
目的探讨雄鼠生殖细胞中是否有Tctex5基因的表达。方法从三株体外培养的雄鼠生殖细胞CRL-1715、CRL-2053和CRL-2196细胞株中提取总RNA和蛋白,采用RT-PCR和Western blot分别从mRNA和蛋白质水平分析基因产物的表达。结果Tctex5基因在CRL-1715、CRL-2053、CRL-2196三株细胞中都有高表达。结论Tctex5基因作为一个未知功能的基因,在雄鼠生殖细胞中有丰富的表达,可能参与了精子发生的功能调节,作为男性正常生育的一个新的标志物,它可能在精子发生中扮演了一个重要的角色,该基因在雄性生殖细胞中的发现可能会对男性不育的诊断与治疗提供新的途径。
Objective To investigate the expression level of Tctex5 gene in germ cells of male mouse. Methods Total RNA and protein was extracted from cultured mouse germ cell lines CRL-1715, CRL-2053 and CRL-2196. The level of messenger RNA and protein of Tctex5 in three cells were used to detect by RT-PCR and Western blot, respectively. Results Tctex5 gene was shown by RT-PCR and Western blot to be expressed abundantly in the three cell lines. Conclusion Tctex5, a gene with unknown function, may involve in the sperm function control process during spermatogenesis, Therefor, Tctex5 may play an important role in spermatogenesis or serve as valuable markers of fertility status. Furthermore, it may be important for diagnosis and therapy of male sterility.
出处
《中国男科学杂志》
CAS
CSCD
2008年第4期5-8,共4页
Chinese Journal of Andrology
