摘要
目的:探讨精液沙眼衣原体(Chlamydia trachomatis,CT)感染与精子凋亡的关系,为研究男性不育提供新思路。方法:沙眼衣原体的检测用免疫层析法,采用瑞-姬染色精子,观察精子形态变化;用末端脱氧核苷酸转移酶(TdT)介导的脱氧核苷酸原位末端标记法(TUNEL)检测凋亡精子。结果:精液CT阳性组精子凋亡率(33.03±14.98)%,正常对照组精子凋亡率为(9.68±2.78)%,组间差异显著(P<0.01)。结论:精液CT感染导致精子凋亡率增加,CT感染可能是男性不育的重要原因之一。
Objective： To investigated the relationship of Chlamydia trachomatis （CT） infection with sperm apoptosis that might relate with male sterility. Method： Immunochromatography method was used to detect Chlamydia trachomatis; Conventional cell morphology staining （wright-gimsa staining）, and terminal deoxynucleotidyl transferase enzyme （TdT） mediated deoxynucleotidyl in situ and labeling （TUNEL） were adopted for apoptosis detection. Results： Sperm apoptosis rates were 33. 03± 14. 98% and 9.68±2.78% in CT infection group and normal fertility group respectively; There was dramatically difference between the two groups （P 〈 0.01 ） , which showed that the CT infection of semen caused increase of sperm apoptotic rates. Conclusions： CT infection might be one of the most important causes for male infertility.
出处
《贵阳医学院学报》
CAS
2009年第3期286-289,共4页
Journal of Guiyang Medical College
基金
贵阳市科技局基金(T2007-7)
贵阳医学院青年教师基金(K2007-23)
黔科合S字(2007)1030
黔科教办(2007)03号
关键词
精子
衣原体
沙眼
细胞凋亡
不育
男性
spermatozoa
chlamydia trachomatis
apoptosis
infertility, male
