摘要
为寻找一种合适的提取小鼠尾部基因组DNA的缓冲裂解液,参照分子克隆实验指南提供的裂解液配比,在5个等体积中分别加入1,2,3,4,5 mL的相同质量浓度Tris-Cl和SDS溶液,分析其对小鼠尾部组织提取DNA数量和质量的差异.结果表明,动物组织DNA的提取与组织消化有很大的关系,低质量浓度的Tris-C1只能基本满足实验要求,而若想获得大量的DNA,并且在实验材料较少的情况下,高质量浓度体系将更加优越.而SDS是用于沉淀蛋白质及RNA分子等不稳定的大分子,低质量浓度的SDS基本满足实验要求,质量浓度过大会影响获得高质量浓度的DNA.
In order to find proper cell-lysis buffers of extracting DNA from the mouse tail, we follow the provided solution in the molecular cloning. In the same volume, we add 1, 2, 3, 4,5 mL solution of Tris-Cl and SDS in the same concentration, respectively, analysis the effect of different concentration of Tris-Cl and SDS on extracting DNA from the mouse tail. The result demonstrates that the tissue digestion has great effect on extracting DNA from the mouse tail, and the low concentration of Tris-Cl is good enough for experiments, hut if you want to obtain more DNA, especially on the lack of materials conditions, the high concentration will he better. Moreover, the SDS is used to deposit the big and unstable molecules, such as protein and DNA, the low concentration of SDS is enough, reversely, the high concentration will affect the purity of the DNA.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2008年第2期192-195,共4页
Journal of Henan Agricultural University
基金
教育部科学技术研究重点项目(206083)
河南省教育厅高校杰出科研人才创新工程项目(0006KYCX015)
