摘要
对NDV不同基因型毒株HN基因的氨基酸序列比较后发现，基因Ⅶ型的毒株在第65～75位的氨基酸序列较为保守，而其他各基因型在该区域则不尽相同。克隆了NDV基因Ⅱ、Ⅶ、Ⅸ型的代表毒株LaSota、GX-2、F48E。的含有该区域的序列，并进行蛋白表达，通过特异性抗血清对表达肽段进行抗原性测定。应用表达的蛋白免疫SPF鸡，用EuSA检测各组的抗体水平。结果表明3个毒株在反应原性上存在差异。应用NDVF48E9强毒株攻毒，结果显示各多肽蛋白的保护率不同，表明在该区域这3个毒株之间存在着免疫原性差异。
After comparisons of the sequences of amino acids of HN gene among NDV strains with genotype difference, it was revealed that in strain Ⅶ the sequence of amino acid from 65 to 75 was conservative, while the same fragments in other strains were different from each other. So the fragments of HN gene in strain La Sota, GX -2 and F4sE9 ,which were the representative strains of Ⅱ、Ⅶ、Ⅸ genotype of NDV, were cloned and expressed, while the antigenicities of expressed peptides were assayed by anti-serum. SPF chickens were immunized by each expressed proteins and the antibody titers of different groups were assayed by ELISA. The results showed that there were differences in reactionogenicity in 3 strains. All chickens which were immunized by different expressed proteins were challenged by F4s E9, a velogen strain of NDV. Results showed that the protection rate of every expressed protein was different from each other,which illustrated that there were differences of immunogenicity in researched region among 3 strains.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第5期93-98,共6页
China Biotechnology
关键词
新城疫病毒
血凝素讲经氨酸酶融合蛋白
免疫活性
Newcastle disease virus Hemagglutinin-neuraminidase fusion protein Immunogenicity
