摘要
通过对NDV不同基因型毒株HN基因的氨基酸序列进行比较，发现基因Ⅶ型的毒株在第65～75位的氨基酸序列较为保守，而其他各基因型在该区域则呈现出多态性。因此克隆了新城疫基因Ⅱ、Ⅶ和Ⅸ型的代表性毒株La Sota、GX-2、F48E9的含该区域的序列，分别将其连接到原核表达载体pGEX-6p-1上，通过特异性抗血清对表达肽段进行抗原性测定。结果表明3个毒株在免疫活性上存在差异，分析其原因可能是由于氨基酸的极性不同所致。
The conservative region of three different genotypes of Newcastle Disease Virus （ NDV ） was found by comparing the HN protein of different strains, while the other genotypes presented variable polymorphic forms. The fragments of three genotypes HN gene （ Ⅱ , Ⅶ, Ⅸ ） comprising the conservative region were cloned into the pGEX-6p-1 expressing vector. The antigenicity of the expressed peptides was identified by Westen-blot. Result shows that there are differences between the three strains in immunogenicity, which may be caused by the variance in the polarity of amino acids.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2007年第4期66-68,共3页
Journal of Northeast Forestry University
基金
国家高技术研究发展计划"863"计划项目(2003AA241110)。
关键词
新城疫病毒
融合血凝素-神经氨酸酶蛋白
反应原性
Newcastle disease virus
Fusion hemagglutinin-neuraminidase protein
Reaction activity
