摘要
目的:观察单侧输尿管梗阻(unilateral ureteral obstruction,UUO)大鼠模型中PTEN在肾小管中的表达;动态观察其与肾间质纤维化程度的关系。方法:制备UUO大鼠模型,采用免疫组织化学检测UUO组及假手术组(sham-operat-ed rates,SOR)术后第7天、14天、21天肾小管中PTEN的表达及其与肾间质纤维化程度的关系。结果:UUO组术后第7天、14天、21天与SOR组相比PTEN的表达减弱(P<0.05),第21天PTEN的表达明显弱于第7天、14天的表达(P<0.05)。结论:PTEN可能参与了UUO大鼠肾间质纤维化的发病过程。
Objective：To explore the role of PTEN in the renal tubular in the process of renal interstitial fibrosis caused by unilateral ureteral obstruction（UUO） in rats. Methods： Rats were sacrificed at the 7th, 14th and 21th days after UUO or shamsurgery. The protein expression of p27 in renal tubular was detected by immuohistochemistry at each time point （the 7th, 14th,21st days）. Results：At the 7th, 14th and 21th days after surgery, there was a significant decrease in expression of PTEN in renal tubular cells between UUO and SOR（ P 〈 0.05）. The expression of PTEN in renal tubular cells was noticeably changed at the 21st days after UUO. Conclusion：The PTEN expression was obviously decreased in rats after UUO, and it was significantly reduced with the renal interstitial fibrosis progressing.
出处
《中国中西医结合肾病杂志》
2008年第5期408-409,I0008,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
关键词
PTEN
输尿管梗阻
纤维化
肾脏
PTEN
Unilateral ureteralobstruction
Fibrosis
Kidney
