摘要
目的:研究单侧输尿管梗阻(unilateralureteralobstruction,UUO)大鼠肾小管上皮细胞p27的表达与肾间质纤维化的关系,并探讨血管紧张素转化酶抑制剂依那普利干预后p27表达的变化,以进一步阐述其抗纤维化机制。方法:90只SD大鼠随机分成假手术组(sham-operatedrates,SOR)、UUO模型组、依那普利治疗组(UUO+Enalapariltreatedgroup,T-UUO)。于术后第7,14,21天分别处死各组大鼠10只。用HE染色动态观察肾脏病理变化,免疫组织化学法测定p27的表达及动态变化,RT-PCR法测定p27mRNA的水平。结果:SOR组肾小管上皮细胞p27蛋白及肾皮质p27mRNA的表达均强,UUO组随着间质纤维化程度的加重,p27mRNA的表达逐渐减弱,而依那普利在UUO术后第14,21天能增强p27的表达。结论:(1)p27参与了UUO大鼠肾间质纤维化的发病过程;(2)UUO大鼠肾间质纤维化模型中依那普利抗间质纤维化机制部分是通过增强p27的表达。
Objective To explore the effect of p27 in the renal tubule on the process of renal interstitial fibrosis caused by unilateral ureteral obstruction （UUO） in rats, and to examine the expression changes of p27 after enalapril intervention and to interpret the anti-fibrotic mechanism. Methods Ninety rats were randomly divided into the sham-operated group （SOR）, UUO group, and UUO ＋ enalapril treatment group [ enalapril： 10 mg/（ kg·d ） ]. The rats of each group were respectivedly sacrificed on 7, 14, 21 days post-operatively. The renal pathological changes were dynamically observed by HE . The expression and dynamic changes of p27 were detected by immunohistochemistry. The level of p27 mRNA were detected by RT-PCR. Results The expression of p27 in renal tubular epithelial cells and p27 mRNA were strongly positive in the SOR group. With degree of interstitial fibrosis aggravating, the expression of p27 mRNA was gradually reducing. Enalapril could improve the expression of p27 on the 14th and 21st days after the UUO. Conclusion （ 1 ） This study supports a causative role of p27 in the formation of fibrosis of renal mesenchyme in rats with UUO. （2） The anti-fibrotic mechanism of enalapril is partly the improvement of p27 expression.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期671-675,共5页
Journal of Central South University ：Medical Science
