摘要
学术背景:近年来人们从神经系统中分离出来的神经干细胞作为神经细胞和神经胶质细胞的共同前体细胞,不仅具有极强的自我更新和增殖能力,同时在神经损伤的修复过程中也发挥着极其重要的作用。目的:总结神经干细胞移植对缺氧缺血性脑病的修复作用。检索策略:主要文献由该论文的研究人员应用计算机检索Pubmed数据库1993/2007年相关文献,检索词"Neural stem cells,brain,hypoxic-ischemic",并限定文章语言种类为English。共检索到131篇文献,对资料进行初审,纳入标准:①与神经干细胞修复脑损伤密切相关。②同一领域选择近期发表或在权威杂志上发表的文章。排除标准:重复性研究。文献评价:文献的来源主要是神经干细胞的相关研究以及修复缺氧缺血性脑损伤方面的随机对照试验。所选用的33篇英文文献中,分别涉及神经干细胞的生物学特性、分布、分离培养以及对缺氧缺血性脑损伤修复的基础实验和相关临床研究。其中7篇为综述,其余均为临床或基础实验研究。资料综合:研究表明,移植后的神经干细胞不仅可以在受体内存活,而且可以进一步增殖、分化,完成受损部位结构和功能的修复。关于神经干细胞在脑损伤修复方面的研究虽已取得较大进展,但仍存在许多问题,如供体细胞的来源、保存,细胞体外培养中分化增殖的调控机制不十分清楚,产生的神经细胞的数量和功能活性不太满意,修复组织内细胞分子生物学特性还不完全明确等。结论:神经干细胞作为理想的种子细胞,为修复缺氧缺血性脑损伤提供了机会窗口,开启了临床应用干细胞治疗的时代。
BACKGROUND： Recently, researchers separate neural stem cells （NSCs） from neural system. As the progenitor of nerve cell and glia cells, NSCs have strong ability of self-renewal and proliferation, and play a very important role in repair process after nerve injury.
OBJECTIVE： To review the repairing effect of NSC transplantation on hypoxic-ischemic encephalopathy.
RETRIEVAL STRATEGY： Using the keywords ＂Neural stem cells, brain, hypoxic-ischemic＂, we searched PubMed database for related articles published in English between 1993 and 2007. 131 articles were collected, and after the first trial, only articles （1） related to neural stem cell transplantation for brain injury, and （2）published in recently or in authoritative journals were selected. Repetitive researches were excluded.
LITERATURE EVALUATION： The articles were about the studies of NSCs and randomized and controlled experiment for hypoxic-ischemic brain injury. Thirty-three English articles were included involving the biological characteristics of NSC, distribution, separation and culture in vitro, and the basic experiment or clinical research about the repair of hypoxic-ischemic brain injury by NSC. Of these articles, there are 7 review articles, and the others were about clinic or basic experiment researches.
DATA SYNTHESIS： Researches show that after transplantation NSC can survive in host, furthermore, it proliferates and differentiates to repair the configuration and function of injured brain area. Even though we have achieved much in NSC transplantation for brain injury, yet there are many problems to be resolved, such as NSC source and conservation, the control mechanism of differentiation and proliferation when NSC is cultured in vitro. In addition, the amount or functional activity of NSCs acquired is lower, and the molecular biological features of cells in repaired tissue are uncertain.
CONCLUSION： As a kind of ideal seed cell, NSC provides an approach for the repair of hypoxic-ischemic brain injury, and sta
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第16期3144-3147,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
