摘要
用聚合酶链反应与酶切技术相结合，对１０例重叠甲型肝炎病毒（ＨＡＶ）感染的慢性乙型肝炎病毒（ＨＢＶ）感染者进行了前Ｃ区Ａ１８９６终止变异和Ｃ区Ｌ９７（异亮氨酸→亮氨酸）变异的动态检测。结果：重叠感染组第一次ＰＣＲ检测ＨＢＶＤＮＡ和Ａｌ８９６、Ｌ９７变异检出率分别为１０％、１７％和１０％，明显低于单独ＨＢＶ感染组的６５％、６８％和５４％（Ｐ＜０．００１），结果提示，重叠ＨＡＶ感染可对ＨＢＶ产生抑制作用，肝损害主要与ＨＡＶ感染有关。
1896 stop mutation of precore gene and L97 (isoleucine leucine)mutation of core gene in 10 cases of patients with chronic hepatitis B super infection hepatitis A virus (HAV) were dynamically detected by combining polymerase chain reaction (PCR) with restriction fragment length polymorphism.The present rate of HBV DNA of lst PCR and mutations of A1896 and L97 in superinfection group is 10%,17% and 10% respectively,which is significantly less than 65%.68% and 54% of HBV infection alone respectively (P<0.01).The results suggest superinfection HAV might take suppressional role on HBV,the liver demage is largely due to HAV infection.
出处
《临床肝胆病杂志》
CAS
北大核心
1997年第4期206-208,共3页
Journal of Clinical Hepatology
