摘要
为研究酪蛋白酶解物对宫内生长迟缓(IUGR)仔猪肠道生长发育的影响,本试验选择15头IUGR新生仔猪,分为新生对照组(N组)、酪蛋白组(C组)和酪蛋白酶解物组(CH组),以牛乳为基础日粮,C和CH组分别加入10%的酪蛋白溶液和10%的酪蛋白酶解物溶液,人工饲喂3 d后取样。结果显示,CH组仔猪小肠长度显著高于C组(P<0.05),CH组空肠后段和回肠前段的绒毛高度/隐窝深度显著高于C组(P<0.05);CH组小肠黏膜的蛋白质含量显著高于C组(P<0.05),同时其DNA含量显著高于C和N组(P<0.05);CH组的麦芽糖酶活性和相对活性均显著高于C和N组(P<0.05或P<0.01),CH组的碱性磷酸酶(AKP)活性显著高于C组(P<0.05)。结果表明,酪蛋白酶解物有促进新生IUGR仔猪肠道组织生长的作用,同时能够刺激IUGR仔猪小肠黏膜上皮细胞的增殖和提高小肠黏膜麦芽糖酶和碱性磷酸酶的活性。
The effects of casein hydrolysate on the growth and development of intestine in intrauterine growth restriction （IUGR） piglets were examined in this study. Fifteen piglets were selected and divided into newborn control group （N）, casein group （C） and casein hydrolysate group （CH）. The cow-milk was taken as the basic feed, piglets in group C and CH were fed milk substituted casein solution and casein hydrolysate solution for 10% milk, respectively. The piglets were slaughtered and sampled 3 days after feeding. The intestinal length of piglets in group cH was significantly higher than that in group C （P〈0.05）. The villus height/crypt depth of distal jejunum and primal ileum in group CH was significantly higher than that in group C （P〈0.05）. The protein and DNA contents in intestinal mocosa in group CH were significantly higher than those in group C （P〈0.05）. The DNA concentration in intestinal mocosa in group CH was higher than that in group C （P=0. 056）. The activity and relative activity of maltase in intestinal mucosa in group CH were significantly higher than those in group C and N （P〈0.05 or P〈0.01）. The activity of AKP in intestinal mucosa in group CH was significantly higher than that in group C （P〈0.05）. These results suggested that casein hydrolysate can stimulate the hyperplasia of epithelium cell and increase the activities of maltase and AKP in small intestinal mucosa.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第5期594-600,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(3077156930471253)
关键词
宫内生长迟缓
仔猪
酪蛋白酶解物
肠道
生长发育
intrauterine growth restriction
piglet
casein hydrolysate
intestine
growth and development
