摘要
目的:研究表皮生长因子(EGF)对人肺癌细胞系A549细胞在体外直流电场中定向移行的促进作用。方法:按A549肺癌细胞是否暴露于直流电场及是否给予EGF分为A、B、C、D4组。显微摄像系统每15min连续记录2h内细胞移行的变化图像,测量细胞移行距离及细胞移行方向与电场方向间夹角cosineγ值,Image-ProPlus5.0软件处理结果;Westernblot检测各组细胞中p-Akt及p-Stat3的表达量。结果:未暴露于直流电场中的A、B组细胞无序随意的布朗运动,暴露于直流电场中的C、D组细胞在观察期间呈现向阴极方向定向移行,D组细胞的定向移行cosineγ值最大0.83±0.03,P<0.05;2h后D组细胞定向移行距离更大(63±16.9)μm,P<0.05。Westernblot检测提示电场及EGF均促进p-Akt及p-Stat3的表达,电场及EGF共同作用组其表达最强。结论:体外直流电场可诱导人肺癌细胞系A549细胞朝向阴极的定向移行,EGF可以促进这种定向移行,肺癌细胞在直流电场中定向移行可能与Akt及Stat通路有关。
OBJECTIVE：To study the effect of EGF（epidermal growth factor） on migration behavior of A549 cells in the direct-current electric fields. METHODS： The A549 cells were divided into 4 groups according to whether applying EGF or direct- current electric fields. The movement images were taken per 15 rain within 2 hours by micro-videotape system. Total length of cell displacement and average directional cosine γ were measured. The data of results were analyzed statistically. Western blot method was used to determine expression of p-Akt and p-Stat 3 protein. RESULTS： Cells in group A and B unexposed to the electric fields showed random movements during 2 hours. Cells in group C and D exposed to the electric fields migrate to the cathode direction. The average directional cosineγ of the cells in group D was larger than that in other groups 0. 83 ± 0.03, P〈 0.05. The total length of cell displacement in group D was longer than that in other groups also（63±16.9） μm,P〈0. 05. The expression of p-Akt and p-Stat 3 protein was increased with the treatment of EGF and electric fields. CONCLUSIONS： Electric field is able to induce the cathodal directed migration of the A549 cells and moreover the EGF can enhance this directed migration. We believed that Akt and Star signal conduction path might play a very important role in the directed migration of the A549 cells.
出处
《中华肿瘤防治杂志》
CAS
2008年第8期561-564,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金项目(30670510)
关键词
肺肿瘤
表皮生长因子
电刺激疗法
细胞
培养的
lung neoplasms
epidermal growth factor
electric stimulation therapy
cell, cultured
