摘要
采用紫外线照射的方法对巴氏杜氏藻Dunaliella bardawilH-42(简称H-42)进行诱变.通过小剂量乙醚抽提、索氏法提取和氯仿提取分析,得到两株总脂含量显著高于原出发株的突变株,分别命名为D.bardawilH-42 var.HL-1(简称HL-1)和D.bardawilH-42 var.HL-2(简称HL-2).研究表明,HL-1和HL-2的总脂含量可达细胞干质量的21.1%和20.5%,分别比对照提高了31.1%和27.3%.初步分析了HL-1和HL-2的生物质油主要组分,它们的氯仿沥青"A"(相当于原油)含量分别比对照提高了10.6%和11.8%,具有良好的生物质能源开发前景.随机扩增多态性DNA(RAPD)分析表明:HL-1和HL-2与H-42的遗传相似系数为分别为0.797和0.718,由此确认HL-1和HL-2均为H-42的高脂突变株.
Two mutant strains of Dunaliella bardawil. H-42 （H-42）, with the character of high lipid-content, have been isolated and identified after ultraviolet radiation. They were denominated as D. bardawil H-42 var. HL-1 （HL-1）and D. bardawil H-42 var. HL-2（HL-2） ,respectively. Total lipid contents were determined by the Soxhlet procedure. Contents and the main compositions of biodiesel of the two mutant strains were analyzed by geochemistry experimental technology. The results showed that total lipid contents of HL-1 and HL-2 were 21.1% and 20.5 % （dry mass） ,which were increased as high as 31.1% and 27.3 %,respectively. The RAPD test revealed that the genetic similarity between H-42 and its mutant strain HL-1 was 0. 797, while H-42 and HL-2 was 0. 718. Quantities of the chloroform extracts ＂A＂ from high lipid mutants HL-1 and HL-2 were enhanced more than 10% to their original species H-42. So the evidence mentioned above proved that both HL-1 and HL-2 were experimentally tested to be the mutant strains derived from H-42 and provided a new character of high content of lipid. They may be very good candidates for source of oilproducing microalgae.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期397-401,共5页
Journal of Xiamen University：Natural Science
基金
福建省重大专项前期研究项目(2005YZ1022)资助
关键词
杜氏藻
诱变
脂类
生物质油
氯仿沥青“A”
Dunaliella bardawil
mutant
lipid content
biodiesel
chloroform extracts ＂A＂
