摘要
目的：检测比较蚊体内的黄病毒。方法：采用RT－PCR技术及常规的C6／36细胞培养结合免疫荧光法。结果：无论实验感染的登革热病毒蚊虫标本，还是从野外捕捉的标本，均表明RT－PCR检出黄病毒的阳性率比常规法高，且快速、敏捷，操作简便；常规法检出周期长（2～3周），操作复杂、繁琐和反复多次，检出率低。但常规法能通过细胞病变，观察病毒的毒力，这是RT－PCR所不及的。结论：以RT－PCR结合常规法进行虫媒病毒的研究，将更有利于探索蚊虫与虫媒病毒的关系和内在规律。
We have made a comparison for detecting Flaviviruses in mosquitoes between reverse transcription-polymerase chain reaction (RT-PCR) and C6/36 cell culture-McAb-IFA (conventional method). The results showed that the pasitive rates of flavivirus infection by RT-PCRwhether Dengue viruses infected mosquitoes in laboratory or naturally catched mosquitoes werehighter than that by conventional method. The duration of detected Flavivirus infection was shorterby RT-PCR than by conventional method and the operation was simpler. But toxicity of flaviviruscould be observed through cells pathological changes for the latter and it not for the former.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1997年第6期444-446,共3页
Chinese Journal of Vector Biology and Control
