摘要
用ＢａｍＨＩ和ＨｉｎｄＩＩ将丙肝病毒Ｃ＋Ｅ１ＤＮＡ片段从其克隆载体ｐＧＥＭ３ｚｆ－ＨＣＶ／Ｃ＋Ｅ１上切下，经Ｔａｑ酶补齐３’末端后插入到载体ｐＳＶＬ－Ｔ中，构建成丙肝病毒Ｃ＋Ｅ１真核表达载体ｐＳＶＬ－ＨＣＶ／Ｃ＋Ｅ１。本实验中重组效率达６４．７％（１１／１７），正向插入为５０％（２／４）。
The clone plasmid of hepatitis C virus /C+E1 was digested with the restriction enzymes to get the HCV/C+E1 DNA fragment.The single 3'-A was added to C+E1 DNA by Taq DNA polymerase to form C+E1-A DNA fragment.The expression vector of hepatitis C virus/C+E1(pSVL-HCV/C+E1)was constructed by inserting the C+E1-A DNA into pSVL-T eukaryotic vector.In this experiment,the recombinant efficent of C+E1-A and pSVL-T vector was 64.7%(11/17),and the positive-orientation pSVL-HCV/C+E1 reached 50%(2/4).The pSVL-HCV/C+E1 can cause BALB/C mice to produce antibodies against HCV/C.
出处
《微生物学免疫学进展》
1997年第4期25-27,共3页
Progress In Microbiology and Immunology
