摘要
将anti-ras核酶基因R8克隆于不同的逆转录病毒载体,并导入逆转录病毒包装细胞系PA317,得到具一次感染性的缺失性病毒,通过测定病毒滴度选择R8基因的高效重组逆转录病毒载体.
A series of recombiant retroviral vectors which contain riobzyme R8 gene were constructed, then transfected packing cell lines PA317 to get retrovial vector producer cell clones. The most effcient vector was got by testing the titer of the recombinat virus.
出处
《南开大学学报(自然科学版)》
CAS
CSCD
北大核心
1997年第4期8-13,共6页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
天津市21世纪-青年科学基金