摘要
以大肠杆菌表达的犬瘟热病毒(CDV)重组核蛋白(GST-NP)经纯化后作为包被抗原,通过方阵ELISA滴定确定GST-NP的适宜包被浓度为1.31μg·mL-1,并由此建立检测抗CDV抗体的间接ELISA方法。通过对已知抗CDV阳性血清及抗其他病毒血清的试验,表明所建立的间接ELISA可特异检测动物血清中的抗CDV抗体。另外,通过不同样品的重复试验以及不同批次的检测试验,证明该方法在用于检测CDV抗体时具有很好的重复性和稳定性。
The recombinant nucleocapsid protein of canine distemper virus （CDV） expressed in Escherichia coli BL21 was used as antigen for developing an indirect-ELISA assay to detect CDV specific antibody. Conditions of the ELISA were optimized, and the optimistic concentration of the recombinant nucleocapsid protein was 1.31 μg · mL^-1. Different serum samples were detected for the antibody of CDV, and it was found that the developed ELISA method was sensitive and specific to the positive serum of CDV, while not reactive to the negative serum of CDV and the sera of other virus, such as Canine parvovirus and canine parainfluenza virus. In addition, the data of further tests also confirmed the ELISA method was repeatable within subject and stable between subject.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2008年第1期31-33,55,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省高校自然科学基金资助项目(VK0410082)
关键词
犬瘟热病毒
抗体
间接ELISA
检测
canine distemper virus
antibody
indirect-ELISA
detection
