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传染性法氏囊炎病毒(IBDV)血清Ⅰ型和血清Ⅱ型抗体的鉴别检测 被引量:1

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摘要 利用血清中和试验(VN),酶联免疫吸附实验(ELISA)和琼脂扩散沉淀试验(AGP)对IBDV血清Ⅰ型和血清Ⅱ型抗体进行鉴别检测。被检血清来自用活毒攻毒鸡和用灭活油佐剂疫苗免疫鸡,血清Ⅰ型和血清Ⅱ型两者之间抗体的差异用VN试验很容易鉴别检出,但用ELISA方法却不能区分两者之间的差异。AGP试验更不能令人满意。目前用于检测IBDV抗体水平的方法主要有VN、AGP和ELISA等。VN试验不仅可鉴别检测血清Ⅰ型和血清Ⅱ型IBDV抗体,而且可鉴别检测同一血清型内不同毒株的抗体。ELISA也同样用于检测鸡的IBDV抗原和抗体,并被广泛应用于测定大型鸡场IBDV的免疫状态。此报道的主要目的是对比ELISA,AGP试验和VN试验检测IBDV血清Ⅰ型和血清Ⅱ型特异性抗体之间差异的敏感性。
出处 《山东家禽》 1992年第2期35-37,共3页 Shandong Poultry
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同被引文献9

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