摘要
甲基球菌３０２１中的甲烷单加氧酶在甲烷生物催化氧化制甲醇的反应中具有重要的作用．实验结果表明，３０２１菌的热稳定性好于其它甲烷氧化细菌，可在４５℃反应５ｈ．在２００ｍｍｏｌ／Ｌ磷酸缓冲液中，它的甲醇累积速率比甲基弯菌ＩＭＶ３０１１高．乙二胺四乙酸（ＥＤＴＡ）、甲酸钠、Ｎａ－Ｃｌ可明显增加甲醇累积量．另外。
A methanotrophic bacteria, Methylococcus capsulatus IMV 3021 grew in a media with methane as the sole source of carbon and energy. The methane monooxygenase system(MMO) of these cells plays an important role in bioconversing methane to methanol. The biocatalytic and reaction conditions of these resting cells were studied. The high concentration phosphate buffer (200 mmol/L) inhibited MDH activity. The methanol accumulation rate of the resting cells was higher than those of Methylosinus trichosporium IMV 3011 and Methylomonas sp S. The methane monooxygenase thermostability of Methylococcus capsulatus IMV 3021 was better than that of Methylosinus trichosporium IMV 3011. Methanol accumulation still increased after the former reacted 3-4 h at 45℃. Apparently, addition of NaCOOH, EDTA and NaCl stimulated methanol accumulation. Optimum gas composition and reaction temperature were discussed.
出处
《分子催化》
EI
CAS
CSCD
北大核心
1997年第5期349-353,共5页
Journal of Molecular Catalysis(China)
基金
中国科学院"八五"重点应用项目
关键词
甲基球菌
甲醇
甲烷单加氧酶
催化
Methanotrophic bacteria, Methane monooxygenase(MMO), Methanol accumulation, Methanol dehydrogenase(MDH)
