摘要
目的:合成头孢吡肟 E-异构体,并作为工作对照品,建立头孢吡肟中 E-异构体含量的分析方法。方法:采用 RP—HPLC,色谱柱为 Alltima C_(18)(4.6 mm×150 mm,5μm);线性梯度洗脱,流动相 A 为0.057%磷酸二氢铵溶液,流动相 B 为乙腈,25 min 内,流动相 B 由0%增加至25%;流速为1.0 mL·min^(-1);检测波长为254 nm。结果:方法的线性范围为0.2～4.06μg·mL^(-1),线性方程为 Y=-1.3×10~4+9.8×10~4X(r=0.9981),检测限为20.5 ng;平均回收率为99.4%,RSD 1.5%。结论:本法分离效果好,灵敏度高,结果准确、可靠,适合于头孢吡肟中 E-异构体的含量测定。
Objective： To study the synthetic method of cefepime E-isomer and using as work standard, its quantitative method was developed by HPLC in eefepime. Method：The separation was performed on a Alhima C 18 column （4.6 mm×150 mm ,5 μm） ,the mobile phase was composed of 0. 057% ammonium phosphate monobasie and ace-tonitrile with gradient elution. The gradient condition was 0-25 min, aeetonitrile 0%-25%. The flow rate was 1.0 mL·min^-1.The absorbanee was measured at 254 nm. Result：A good linearity was obtained over the range of 0.2-4.06 μg·mL^-1;the regression equation were Y=-1.3× 10^4×9.8×10^4X（r=0.9981）,the detection limit was 20.5 ng;the average recovery was 99.4% , RSD 1.5%. Conclusion：The method is proved to be suitable for detecting the content of eefepime E - isomer,which offered the advantages of speeifity, speed and sensitivity.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第4期613-615,共3页
Chinese Journal of Pharmaceutical Analysis
