摘要
以籼稻重穗型恢复系蜀恢527的成熟胚为外植体,研究了影响其愈伤组织诱导及植株再生频率的各种因素。结果表明,在基本培养基NMB中添加2.0mg/L的2,4-D有利于蜀恢527成熟胚愈伤组织的诱导和继代培养;MS+6-BA2.0mg/L+KT2.0mg/L+NAA0.25mg/L的分化培养基有利于愈伤组织的分化;继代两次的愈伤组织分化率最高。利用本研究建立了适合蜀恢527成熟胚遗传转化的高效植株再生体系,在此基础上利用农杆菌介导法将稻瘟病抗性基因Pi-d2导入蜀恢527并获得了经PCR检测为阳性的转化植株。
Using mature embryos of restorer line Shuhui527 as explants, some factors which were involved in mature embryos callus culture were studied in this experiment. The results indicated that NMB medium with 2.0 mg/L 2,4-D was suitable for callus from mature embryos induction and subculture of indica rice Shuhui527. Meanwhile, the MS ＋ 6-BA2.0 mg/L ＋ KT 2.0 mg/L ＋ NAA 0.25 mg/L medium was advantageous to callus differentiation. Callus after two subculture passages was more effective in inducing Plant regeneration. A highly efficient regeneration system of Shuhui.527 suitable for genetic transformation was established. Shuhui.527 was applied in the genetic transformation by using Agrobacterium-mediated transformation. The result of PCR assay showed that Pi-d2 gene had been integrated into the rice genome.
出处
《西南农业学报》
CSCD
2008年第2期262-266,共5页
Southwest China Journal of Agricultural Sciences
关键词
籼稻
成熟胚
愈伤组织
再生植株
indica rice
mature embryo
callus
plantlet regeneration
