摘要
以超级杂交稻父本0293为材料,利用农杆菌介导基因转化法研究植物抗病基因snc1在超级杂交稻父本中的遗传转化。结果表明:0293成熟胚愈伤组织的最适诱导培养基为NMB诱导培养基,2,4–D和6–BA的最适质量浓度分别为3.0和0.2 mg/L;以LB液体培养的农杆菌转化能力最强,抽真空转化方式获得的抗性愈伤率较高,最适共培养时间为2 d;麦芽糖为抗性愈伤组织分化的最适碳源,NMB+KT 0.4 mg/L+6–BA 2.5 mg/L+NAA 0.5 mg/L+IAA 0.5 mg/L为较适合的分化培养基;通过优化农杆菌介导的遗传转化条件,共获得6株含目的基因snc1的转化植株。
Male parent of super hybrid rice 0293 （0293） was used to study the genetic transformation of super hybrid rice by Agrobacterium-mediated transferring of plant resistant gene sncl （suppressor ofnprl-1, constitutive 1） into rice plant, The results showed that the NMB medium is the optimal medium for callus induction on mature embryo of 0293, the optimal concentration of plant growth substances 2, 4-D and 6-BA are 3.0 mg/L and 0.2 mg/L, respectively; the transferring capability of Agrobacterium in LB liquid medium is found to be the best, and higher rate of resistant callus is obtained by the vacuum transferring under 2 days of co-cultivation; maltose is the optimal carbon source for resistant callus differentiation, and the best differentiation medium is NMB containing 0.4 mg/L KT, 2.5 mg/L 6-BA, 0.5 mg/L NAA and 0.5 mg/L IAA. After optimized the conditions of Agrobacterium-mediated transferring, 6 transformants with the target sncl gene was obtained in this paper.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第3期267-270,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(30970247)
湖南省杰出青年基金项目(11JJ1007)
湖南省高校科学研究重点项目(09A037)
