摘要
目的建立三黄泻心汤的HPLC指纹图谱分析方法,为研究三黄泻心汤的药效物质基础和不同配伍的有效成分变化提供可行手段。方法采用HPLC法建立指纹图谱,选用Shimadzu ODS色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸水溶液(含0.01 mol/L磷酸二氢钾,pH 2.8)梯度洗脱,体积流量为1.0 mL/min,检测波长260 nm。通过对全方与各组成药味的阳性和阴性对照组指纹图谱中各峰相对保留时间的比较分析。进行主要色谱峰的组成药味归属;利用色谱峰保留时间的对比及向全方样品中加入对照品的方法,鉴定相关色谱峰。结果建立了三黄泻心汤的HPLC指纹图谱,10批样品相似度均大于0.92。以黄芩苷为参照峰,标示出32个共有峰,确认了其中11个活性成分峰,并说明了其药材归属。结论该实验方法简便、准确,重复性好.具有良好的精密度和较好的分离效果,为三黄泻心汤及相关制剂的质量控制提供科学依据。
Objective To establish the HPLC fingerprint of Sanhuang Xiexin Decoction and provide a method to study the potential basis and the changing of the chemical component for it in different compatibility . Methods An HPLC method was established with Shimadzu ODS column （150 mm×4.6 mm, 5 μm）, the mobile phase was methanol-water-0.1% phosphoric acid （0. 01 mol/L potassium phosphate monobasic, pH 2.8） as gradient elution, the flow rate was 1.0 mL/min, and the detection wavelength was 260 nm. Through comparing and analyzing the relative retention time of this decoction and of its composition which are positive and negative control fingerprints, the main chromatographic peak origins were confirmed; The correlated chromatographic peaks were identified by contrasting chromatographic peak retention time and adding reference substances to the sample. Results All tested samples contained the 32 common peaks, the relativity of them were analyzed and 11 peaks were indicated. The similarity of ten batches of samples exceeded 0.92. Conclusion This method shows sensitive and good repeatability, all of the contents are separated well. It is used to determine Sanhuang Xiexin Decoction and its relative preparations.
出处
《中草药》
CAS
CSCD
北大核心
2008年第4期524-529,共6页
Chinese Traditional and Herbal Drugs
基金
黑龙江省博士创新基金项目资助项目[2007]
