摘要
目的:探讨T辅助-1型/T辅助-2型(Th1/Th2)细胞因子与自然流产的相关性及其影响因素,观察补肾健脾中药复方助孕3号方对自然流产患者Th1/Th2细胞因子的影响。方法:采用放免法检测15例正常组妊娠妇女、30例治疗组自然流产患者治疗前后血清IL(白介素)-2、IL-4、IL-10水平,ELISA法检测其血清IFN-γ(γ-干扰素)水平,比色法检测其血清NO(一氧化氮)含量。结果:与正常组相比.治疗前治疗组血清IL-10水平明显下降(P<0.05),IFN-γ/IL-10明显升高(P<0.05),Th1/Th2明显升高(P<0.05)。治疗组自然流产次数与IL-2/IL-4、IFN-γ/IL-4呈正相关(P<0.01);NO与IL-4成负相关(P<0.05).而与IL-2/IL-4、IFN-γ/IL-4均呈正相关(P<0.05)。治疗组治疗后血清IL-2、IFN-γ含量均较治疗前有所下降(P<0.05),IL-2/IL-10比值明显下降(P<0.05),Th1/Th2亦较治疗前明显下降(P<0.05)。结论:自然流产患者存在较高的Th1反应,而较高的NO的表达促进了Th1反应;助孕3号方能有效调节自然流产患者Th1/Th2细胞因子的平衡,并降低NO表达,有利于妊娠维持。
Objective:To explore the correlation of cytokine Th1/Th2 with apontancous abortion (SA)and to observe the effect of herbs of tonifying kidney and strengthening spleen on cytokine Th1/Th2.Methods:Serum levels of intedeukin 2 (IL-2),IL-4 and IL- 10 in 15 healthy pregnant women and in 30 SA patients be- fore and after treatment with Zhuyun Preacription No.3 were detected with radioim- munoassay,their serum interferon gamma(IFN-γ)level was detected by ELISA and serum nitric oxide (NO)content by colorimetry.Results:Compared with the healthy pregnant women,serum level of IL - 10 decreased (P<0.05),[FN-γ/IL - 10 ra- tion increased(P<0.05),and Th1/Th2 also iecreased(P<0.05)before treat- ment.The abortion frequency was positively correlated with the ratio of IL- 2/IL- 4 and IFN-γ/IL-4(P<0.01).NO content was negative correlated with IL-4 in SA patients,while positively correlated with IL-2/IL-4 and IFN-γ/IL-4(P<0.05).IL-2 and IFN-γlevels as well as the ratios of IL-2/IL-10 and Th1/ Th2 were decreased in SA patients after treatment (P<0.05).Conclusion:There exists a high reaction of Thl type which is enhanced by high- level NO.Zhuyun Pre- scription No.3 regulates the balance of Th1/Th2,reduce the expression of NO which benefits to the maintenance of pregnancy.
出处
《新中医》
CAS
北大核心
2008年第5期57-59,共3页
New Chinese Medicine
