摘要
目的观察survivin基因mRNA及蛋白在鼻咽癌的表达及其与临床的关系。方法采用原位杂交和免疫组织化学技术，分别检测survivin基因及蛋白的表达情况。结果在64例鼻咽癌中，survivin基因mRNA阳性表达42例（65．6％），其中高表达30例（46．9％）；在有详尽诊疗资料的22例鼻咽癌中，Ⅲ＋Ⅳ期阳性表达率为66．7％，高于Ⅰ＋Ⅱ期的50．0％。Survivin蛋白阳性表达46例（71．9％），其中高表达38例（59．4％）；Ⅲ＋Ⅳ期阳性表达率为61．1％，高于Ⅰ＋Ⅱ期的50．0％。Survivin基因表达与鼻咽癌患者的年龄、性别无相关性（P〉0．05）。30例慢性鼻咽炎患者鼻咽部组织survivin基因mRNA及蛋白阳性表达分别为10例（33．3％）及7例（23．3％），均低于鼻咽癌组，差异均有统计学意义（χ^2=12．04和χ^2==19．57，均P〈0．01）。64例鼻咽癌中survivin基因mRNA及蛋白共同表达阳性者36例（56．2％），经相关检验它们的表达呈正相关（关联系数φ=0．43）。结论Survivin基因表达可能在鼻咽癌发生、发展过程中起一定作用，检测survivin基因mRNA和蛋白在鼻咽癌中的表达对鼻咽癌的诊断、临床分期和预后有参考的价值。
Objective To investigate the relationship between survivin mRNA and protein expression and nasopharyngeal carcinoma（NPC）. Methods Survivin mRNA and protein were detected by in situ hybridization and immunohistochemical S-P staining respectively. Results Among 64 cases of NPC, 42 cases （65.6%） were positive for survivin mRNA expression, 30 cases （46.9%） had high expression level. In 46 cases （71.9%） of NPC were positive for survivin protein expression, and 38 cases （59.4%） had high expression level. In 22 cases of NPC with detailed clinical information, the positive expression rates of survivin mRNA and protein in stages Ⅲ ＋ Ⅳ of NPC were 66.7% and 61.1% respectively, which were higher than those in stages Ⅰ＋ Ⅱ of NPC （50.0% and 50.0% respectively）. There was no significant difference between sravivin mRNA and protein expression regarding age or gender of NPC patients （ all P 〉 0.05）. The positive expression rates of survivin mRNA and protein in chronic nasopharyngitis group were 33.3 % and 23.3% respectively, which were lower than those in NPC group （χ^2 = 12.04, P 〈 0.01 and χ^2 = 19.57, P 〈 0.01, respectively）. In 64 cases of NPC, 36 cases were positive for beth survivin mRNA and protein, and the expression of survivin mRNA and protein showed positive correlation （ φ= 0.43）. Conclusion The expression of survivin gene may play some roles in the pathogenesis of NPC. Detection of survivin mRNA and protein will be helpful for diagnosis, clinical staging and prognosis of NPC.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2008年第2期179-182,共4页
Chinese Journal of Medical Genetics
基金
国家自然科学基金（30460145）
关键词
鼻咽癌
SURVIVIN基因
原位杂交
免疫组织化学
nasopharyngeal carcinoma
survivin gene
in situ hybridization
immunohistochemical staining
