摘要
背景:因心脏结构高度复杂、心肌组织有很高的机械顺应性,心肌组织工程对材料的要求高。以往研究表明Ⅰ型胶原和胶原海绵材料均适宜心肌细胞生长及分化。目的:拟进一步对比观察胶原海绵和温敏型Ⅰ型胶原与骨髓间充质干细胞的相容性,为心肌组织工程选择更理想的载体材料。设计、时间及地点:在解放军第四军医大学西京医院心血管内科实验室2007-03/10完成对比观察设计的实验。材料:胶原海绵由上海其胜制剂有限公司提供;温敏型Ⅰ型胶原由第四军医大学组织工程中心提供。方法:将体外培养的大鼠骨髓间充质干细胞分别复合于胶原海绵和温敏型Ⅰ型胶原材料,共同培养7d,分别于培养第1,3,5和7天取材。主要观察指标:扫描电镜和苏木精-伊红染色观察骨髓间充质干细胞细胞形态及附着情况;MTT检测骨髓间充质干细胞细胞增殖情况。结果:①扫描电镜检测显示温敏型Ⅰ型胶原材料中骨髓间充质干细胞细胞黏附优于胶原海绵材料,细胞在Ⅰ型胶原材料中生长连接成片,表面有大量分泌颗粒。②MTT检测表明在培养第1,3,5,7天温敏型Ⅰ型胶原材料中骨髓间充质干细胞细胞活性(吸光度值)及细胞分裂增殖速度均高于胶原海绵材料(P<0.01)。结论:温敏型Ⅰ型胶原体外与骨髓间充质干细胞的相容性优于胶原海绵。
BACKGROUND： Cardiac structure is extremely complex and muscular tissues show a high mechanical compliance, therefore, myocardium tissue engineering demonstrates a high requirement for the materials. OBJECTIVE： To explore the biocompatibility of collagen sponge or temperature-sensitive collagen type I with bone marrow mesenchymal stem cells （BMMSCs）, and provide an ideal material for myocardium tissue engineering. DESIGN, TIME AND SETTING： A contrast observation was carried out in the Laboratory of Cardiology, Xijing Hospital, the Fourth Military Medical University of Chinese PLA from March to October in 2007. METHODS： The in vitro cultured rat BMMSCs were compounded to collagen sponge and temperature-sensitive collagen type I, respectively, followed by the culture for 7 days. Samples were obtained at 1, 3, 5, 7 days of culture.
MAIN OUTCOME MEASURES： The morphology and adhesion of BMMSCs were observed under scanning electron microscope and hematoxylin-eosin dyeing; the proliferation of BMMSCs was detected by MTTmethod. RESULTS： ①Under scanning electron microscope, the BMMSCs on the temperature-sensitive collagen type I adhered, which were better than those on collagen sponge, these cells grew and connected with each other, with a large amount of secretory granules observed on the sufface.②MTT result showed that, the BMMSCs activity and the speed of cell division and proliferation were all increased on the temperature-sensitive collagen type I material compared with those on collagen sponge material at 1, 3, 5, 7 days of culture （P〈 0.01）. CONCLUSION： The biocompatibility of temperature-sensitive collagen type I with BMMSCs is superior to that of collagen sponge.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第10期1831-1834,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
