摘要
探讨醛固酮（Ａｌｄ）对血管平滑肌细胞蛋白合成、胶原合成及分泌的影响。方法将培养的乳鼠ＶＳＭＣ分成１７组：正常对照组；Ａｌｄ１０－７组；安体舒通（Ｓｐｉ）１０－７ｍｏｌ／Ｌ组；血管紧张素Ⅱ（ＡｎｇⅡ）１０－７ｍｏｌ／Ｌ组；碱性成纤维细胞生长因子（ｂＦＧＦ）１０ｎｇ／Ｌ组；上述浓度的ＡｎｇⅡ和ｂＦＧＦ分别加入不同浓度的Ａｌｄ和Ｓｐｉ（１０－９，１０－８，１０－７ｍｏｌ／Ｌ）组成不同浓度的配伍组。观察３Ｈ－亮氨酸和３Ｈ－脯氨酸参入的影响，以计算对蛋白质和胶原合成的情况。结果Ａｌｄ明显增加ＶＳＭＣ的３Ｈ－脯氨酸参入，并加强ＡｎｇⅡ及ｂＦＧＦ剌激的３Ｈ亮氨酸和或３Ｈ－脯氨酸的参入及胶原分泌。Ｓｐｉ抑制ＶＳＭＣ的３Ｈ－亮氨酸和或３Ｈ－脯氨酸的参入并抑制ＡｎｇⅡ及ｂＦＧＦ的３Ｈ－亮氨酸和３Ｈ－脯氨酸的参入和胶原的分泌。结论Ａｌｄ作为局部因子，通过旁分泌或自分泌参与ＶＳＭＣ的蛋白合成、胶原合成和分泌。
Aim\ To investigate the effect of aldosterone on the the protein,colagen in cultured rat vascular smooth muscle cells.\ Methods\ Protien synthesis was expressed by 3H leusine incorporation,the collagen by 3H proline in coporation in cultured cell.\ The cultured medium were added with the aldosterone(Ald) 10 7 mol/L alone,spironolactone(Spi) alone,angiotensinⅡ(AngⅡ) alone, basic fibroblast growing fortor(bFGF) alone, bFGF+Ald(in various concentrations), bFGF+Spi(in various concentrations).\ Results\ Adding of Ald increased the incorporation of 3H leucin, 3H proline in cultured vascular muscle cells; and increased the collagen secretion induced by simultaneously adding of AngⅡ and bFGF.\ Spi inhibited the incooporation and the secretion of collagen by adding AngⅡ and bFGF as well.\ Conclusion\ Ald involved in the protien synthesis,collagen synthesis and the collagen secretion. It may serve as a local important factor.
出处
《高血压杂志》
CSCD
1997年第3期178-181,共4页
Chinese Journal of Hypertension
关键词
血管平滑肌细胞
醛固酮
螺旋内酯
蛋白合成
vascular smooth muscle cells(VSMC)
aldosterone
spironolactone
protien synthesis
collagen synthesis
collagen secretion
