摘要
目的:建立高效液相色谱法测定肝炎合剂中大黄素的含量。方法:采用Symmetry C18柱(4.6mm×150mm,5μm),流动相为甲醇-0.1%磷酸溶液(85∶15),检测波长为438nm。结果:此方法线性关系良好,平均加样回收率为99.04%。结论:此方法简便、准确,可用于测定肝炎合剂中大黄素的含量。
Objective：To establish a HPLC methed for the determination of emodin in Ganyan Mixtures. Methods： The HPLC method was used at Symmetry Cxs column （4.6 mm ×150 mm, 5 μm）. The mobile phase consisted of methanol - 0. 1% H3PO4 （85 ： 15 ）. The detective wavelength was 438 nm. Results： The linearity of this method was well with the average recovery of 99.04%. Conclusions： The method is simple and accurate. It can be used for the determination of the content of emodin in Ganyan Mixtures.
出处
《药学与临床研究》
2008年第2期155-156,共2页
Pharmaceutical and Clinical Research
