摘要
目的：建立测定海洋抗肿瘤活性成分海参皂苷Nobiliside-A的HPLC含量测定方法。方法：通过比较紫外检测器和蒸发光散射检测器以及不同的色谱柱、不同的流动相系统，确定了Nobiliside-A的HPLC-ELSD含量测定方法，并对线性范围、精密度、稳定性进行了考察。结果：采用蒸发光散射检测器，Agilent Zorbax SB-C18色谱柱（150mm×4．6mm，5μm），流动相为甲醇：0．5％HAC（78：22），在0．273—4．24 μg范围内Nobiliside-A进样量的自然对数与峰面积的自然对数呈良好的线性关系（r=0．9995），日内、日间精密度以及稳定性均达到要求。结论：该实验为海参皂苷Nobillside-A的检测提供了准确、快速的分析方法。
AIM ： To establish the assay method of Nobiliside-A, an anticancer active component in sea cucumber, by HPLC. METHODS： Comparative experiment was carried out between different detector and different chromatographic column and different mobile phase, at last a HPLC-ELSD method was determined, and linear range, precision and stability were studied. RESULTS： ELSD detector and Agilent Zorbax SB-C18 column were used, mobile phase was methonal： 0.5% HAC （78：22） , the natural logarithm of content of Nobiliside-A and the natural logarithm of the peak area were linear in the range of 0. 273 - 4.24 μg （ r = 0. 999 5 ）, stability and precision within day and between day were satisfied. CONCLUSION： The experiment has provided a rapid and precise method for determining the content of Nobiliside-A.
出处
《中成药》
CAS
CSCD
北大核心
2008年第4期567-570,共4页
Chinese Traditional Patent Medicine
基金
“十一五”国家高技术研究发展计划(863计划)(编号:2006AA090304)
