摘要
目的从黑乳海参中分离活性皂苷类成分。方法从黑乳海参体内得到的总皂苷溶于吡啶-二氧六环(1∶1)的混合液中,120℃加热2.5 h,应用多种色谱技术对回流产物进行分离纯化,分离得到2个三萜皂苷类化合物。结果应用现代光谱技术(尤其是2D-NM R和ES I-M S技术)和化学方法鉴定其结构:3-O-〔3-O-甲基-β-D-吡喃葡萄糖(1→3)-β-D-吡喃奎诺糖(1→4)-β-D-吡喃奎诺糖(1→2)-β-D-吡喃木糖〕-海参烷-9-烯-3,β12,α17,α25β-四醇(Ⅰ),命名为黑乳海参皂苷1a;3-O-〔3-O-甲基-β-D-吡喃葡萄糖(1→3)-β-D-吡喃葡萄糖(1→4)-β-D-吡喃奎诺糖(1→2)-β-D-吡喃木糖〕-海参烷-22,25-环氧-9-烯-3,β12,α17α-三醇(Ⅱ),命名为黑乳海参皂苷2a。结论2个化合物均为新化合物。
Objective Holothuria nobilis. Methods Triterpene glycosides were expected to be isolated from the sea cucumber The crude saponins of sea cucumber were desulfated with pyridine-dioxane （1 ： 1）, the desulfated products were separated by multi-chromatography to afford two compounds. Resuits On the basis of chemical methods and spectral evidences, especially 2D NMR and ESI-MS technolo- gies, these two compounds were identified as 3-O-C3′″-O-methyl-β-D-glucopyranosyl （1→3）-β-D- quinovopyranosyl （ 1 →4 ）-β-D-quinovopyranosyl （ 1 →2 ）-β-D-xylopyranosyl]-holosta- 9-ene- 3β, 12α, 17α, 25β-tetraol （ Ⅰ ）, named as nobiliside la; 3-O-（3′″-O-methyl-β-D-glucopyranosyl （1 → 3）-β-D-xylopyranosyl （ 1 →4 ）-β-D-quinovopyranosyl （ 1→ 2 ）-4＇ -O-sulfate-β-D-xylopyranosyl]-holosta- 22, 25-epoxy- 9-ene- 36, 12α, 17α, 25β-triol （ Ⅱ ）, named as nobiliside 2a. Conclusion Two compounds are new triterpene glycosides.
出处
《中草药》
CAS
CSCD
北大核心
2006年第4期497-500,共4页
Chinese Traditional and Herbal Drugs
基金
上海科技发展攻关项目(02DZ19101)
