摘要
目的观察葡萄膜炎患者外周血单个核细胞(PBMCs)Toll样受体9(TLR9)、Toll样受体7(TLR7)mR-NA的表达。方法收集42例葡萄膜炎患者(其中发病与感染相关者12例,与感染无关者30例)和正常对照组(30例)外周血,采用淋巴细胞分离液密度梯度离心法分离PBMCs,逆转录聚合酶链反应(RT-PCR)检测PBMCsTLR9、TLR7 mRNA表达的相对含量。结果与感染相关葡萄膜炎患者PBMCs TLR9、TLR7 mRNA相对含量显著高于与感染无关葡萄膜炎患者和正常对照组(P<0.01),与感染无关葡萄膜炎患者与正常对照组比较TLR9、TLR7 mRNA相对含量差异无统计学意义(P>0.05)。结论TLR9、TLR97的表达在与感染相关葡萄膜炎患者中显著升高,提示感染因素在葡萄膜炎中的作用可能通过TLR9、TLR7介导。
Objective To investigate the expression of TLR9 and TLR7 mRNA of peripheral blood mononuclear cells （PBMCs） in patients with uveitis. Methods Peripheral blood was collected from 42 uveitis patients （12 patients with or related to infection and 30 patients without） and 30 healthy individuals as controls. PBMCs were isolated by Ficoll - Hypaque gradient centrifugation. RT - PCR was adopted to determine the relative content of the expression of TLR9 mRNA and TLR7 mRNA of the PBMCs. Results The relative content of expression of TLR9 mRNA and TLR7 mRNA were significantly upregulated in the patients related to infection compared with that of patients unrelated and controls （ P 〈0.01 ）. There was no statistic significance for the differences in the content of expression of TLR9 mRNA and TLR7 mRNA between patients unrelated to infection and healthy controls （P 〉 0.05）. Conclusion The upregulated expression of TLR9 mRNA and TLR7 mRNA in uveitis patients associated with infection indicates that infective factors might be mediated by TLR9 and TLR7.
出处
《徐州医学院学报》
CAS
2008年第4期222-224,共3页
Acta Academiae Medicinae Xuzhou
