摘要
促使蔗糖进入各种代谢途径的关键酶之一是蔗糖合成酶,它也是蔗糖代谢关键酶中极其重要的一种酶.以甘蔗基因组DNA为模板,用PCR技术分段扩增并克隆了甘蔗蔗糖合成酶(Sucrose synthase)基因片段,并进行序列测序,表明该基因全长约为7·5kb,与LingleS.E等报道的甘蔗蔗糖合成酶(SuSy2)基因序列相似性达到99·5%,推导的氨基酸序列同源性达到100%.该序列包含约1·9kb的上游启动子调控区域,16个外显子,15个内含子,3不翻译区等,开放读码框(ORF)编码802个氨基酸,氨基酸序列中存在糖代谢关键酶家族保守的14-3-3蛋白磷酸化位点Ser/Thr,为进一步在转录水平上阐明蔗糖合成酶的表达调控机制奠定了基础.
Sucrose synthase 2 （SuSγ2） is a key enzyme regulating sucrose metabolism in higher plants, which promotes sucrose into various metabolic pathways. The degenerated oligonucleotides to highly conserved region of SuSγ2 were used to prime the amplification of specific fragment by PCR in samples of sugarcane DNA. One fragment of about 7. 5 kb was cloned and its complete nucleotide sequence was determined. Its nucleotide sequence and the protein from its coding region was 99.5% and 100% identical to the deduced proteins from the sugarcane SuSγ2 cDNA. The sequence also included about 1.9 kb upstream promoter regulation ,sixteen extrons, fifteen introns, 3＇ untranslatded region, and the open read frame coded 802 amino acids. The aminoacid sequence had a conservative sucrose metabolism key enzyme family Ser/Thr bit point. All that laid the foundation for its transcriptional expression regulation mechanism. Fig 2, Ref 19
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2008年第2期177-179,共3页
Chinese Journal of Applied and Environmental Biology
基金
国家“948”项目基金(2006-G37)
农业公益性行业科研专项(ny-hyzx07-019)
福建省教育厅基金项目(JB06091)~~
