摘要
目的探讨慢性乙肝患者外周血单个核细胞(PBMC)APOBEC3G mRNA的水平,以及其与IFN-γ mRNA、血清HBV病毒载量及ALT的关系。方法用实时荧光定量RT-PCR的方法检测27例慢性乙肝患者以及16名健康人PMBC中APOBEC3G和IFN-γ mRNA的水平,并分别检测每个乙肝患者血清HBV病毒载量及肝功能ALT。结果慢性乙肝患者PBMC中APOBEC3G mRNA的表达水平高于健康对照组,差异具有显著性(P<0.01),IFN-γ mRNA无明显增高,A3G还与患者血清中的病毒载量呈正相关(r=0.73,P<0.01),但与IFN-γ及ALT水平无关(P>0.05)。结论表达增高的APOBEC3G蛋白可能只是HBV慢性感染的伴随表现,APOBEC3G蛋白是否具有抗HBV临床应用价值,还需要更加深入的研究探讨。
Objective To assess the level of APOBEC3G mRNA in peripheral blood monouclear cells（PMBC） from chronic hepatitis B patients and to examine the relationship of it with IFN-γ mRNA、HBVviral loads and ALT. Methods real - time fluorescence quantitative RT - PCR was used to quantify APOBEC3G and IFN-γ mRNA levels in PBMC from 16 HBV - uninfected subjects and 27 patients with chronic hepatitis B discease. Besides, HBV viral loads and ALT were detected too. Results APOBEC3G mRNA levels in PBMC of hepatitis B patients were higher than those of HBV - uninfected subjects（P 〈 0. 01 ）,while the levels of IFN -~/ mRNA were not enhanced as compared to HBV -uninfected subjects. Futhermore, the relative level of A3G mRNA were correlative with HBV viral loads directly （r = 0. 73 ,P 〈 0. 01 ）, In Contrast, ALT and IFN -γ mRNA was not relative with APOBEC3G mRNA transcription in vivo. Conclusion The enhanced expression of APOBEC3G might be a concomitant phenomenon of HBV infection. It needs much further investigation to determine whether APOBEC3G proteins have clinical value against HBV infection.
出处
《临床肝胆病杂志》
CAS
2008年第2期83-85,共3页
Journal of Clinical Hepatology
