摘要
针对通常采用的燕麦属牧草染色体观察的去壁低渗法,对该方法中的预处理和解离等环节进行了优化,即预处理用对二氯苯在20℃下处理4 h,固定,酸解离用1 mol/L HCl在60℃下处理10min,酶解离用20 g/L纤维素酶溶液在25℃下处理20 min,最后染色压片。通过研究发现优化后试验步骤简化,成本降低,且观察效果良好。
This study was conducted to optimize both the methods of pretreatment and digestion of the cell wall by enzyme in a wall degradation hypotonic treatment, which was usually accepted to survey chromosome number in oat（Arena）root tip tissue. Basing on the optimized method,the root tip tissues were treated with p-dichlorobenzene for 4 hours at 20 ℃,then fixed the tissues. After being digested by 1 mol/L HCi for 10 minutes at 60 ℃,they were digested by the 20 g/L cellulolyic enzyme for 20 minutes at 25℃. The last step was dyeing and pressing the slide. Since the hypoosmotic step in usual wall degradation hypotonic treatment method was omitted,and the two enzyme digestion steps including pectotytic and cellulolyic enzyme were combined into a cellulolyic enzyme digestion, the method was simplified and its cost reduced. By so doing, an accepted method of chromosome observation for oat root tip tissue was obtained.
出处
《草原与草坪》
CAS
2008年第2期23-26,共4页
Grassland and Turf
基金
国家"十一五"科技支撑计划子课题(2006BAD16B02-2)
关键词
燕麦属
根尖
染色体
去壁低渗法
Arena
root tip
chromosome
wall degradation hypotonic treatment method
