摘要
目的:建立大鼠Hertwig's上皮根鞘细胞分离、培养和纯化的方法。方法:分离出生后7dSD大鼠上下颌第一、二磨牙牙胚,切取牙冠颈部组织,酶消化法原代培养,利用差速传代纯化上皮细胞。用角蛋白14和波形丝蛋白染色确定细胞来源。结果:原代培养细胞为上皮和间充质混合细胞,经2～3次差速传代后可获得纯化的上皮根鞘细胞。免疫组化染色角蛋白14阳性,波形丝蛋白阴性。结论:利用酶消化法及差速传代法培养并获得纯化的上皮根鞘细胞。
Objective：To develop a culture method of Hertwig＇s epithelial root sheath cells. Method：Separate the upper and lower first and second molar germs of post natal 7days SD rats. Cut the cervical loops of these germs and culture the mixed cells by the digestive method. Then,the epithelial cells were purified by several differential passages and identified by immunocytochemical staining of cytokeratin14 and vimentin. Result：The primary cells were mingled by epithelial and mesenchymal cells. After 2～3 differential passages,the cultured cells were turned into purified epithelial cells which were positive stained for cytokeratin14 and negative for vimentin. Conclusion：Hertwig＇s epithelial root sheath cells can be cultured by the digestive method and purified by differential passages.
出处
《临床口腔医学杂志》
2008年第3期142-144,共3页
Journal of Clinical Stomatology
基金
国家自然科学基金面上项目青年基金(30600709)
陕西省科技计划-社发攻关项目2006k11-G1(2)
