摘要
目的比较胰甘油三酯脂酶在1型星形胶质细胞(T1A)和2型星形胶质细胞(T2A)中的表达情况。方法取新生大鼠脑皮质,体外分离培养纯化的T1A和T2A,应用逆转录聚合酶链反应(RT-PCR))技术检测胰甘油三酯脂酶mRNA在两型星形胶质细胞中的表达差异,并且通过激光共焦免疫荧光双标技术从蛋白水平进一步证实。结果胰甘油三酯脂酶在T1A中不表达或低表达,主要定位于细胞核中;而在T2A中表达水平较高,分布广泛,在胞浆、胞核及细胞突起中均有表达。结论胰甘油三酯脂酶在两型星形胶质细胞中的表达存在差异,胰甘油三酯脂酶在T2A中高表达,提示T2A在中枢神经系统脂代谢方面起重要的作用。
Objective To compare the expression of pancreatic triglyceride lipase in type-1（T1A） and type-2 astrocytes（T2A）.Methods Purified cultures of cortical T1A and T2A were prepared from neonatal rats.The expression of pancreatic triglyceride lipase mRNA was detected by reverse transcriptase polymerase chain reaction（RT-PCR）.And the protein level was determined by immunocytochemistry with laser scanning confocal microscope.Results RT-PCR showed that the expression of pancreatic triglyceride lipase mRNA was negative in T1A and positive at a high level in T2A.Immunocytochemistry demonstrated that pancreatic triglyceride lipase expressed at a low level in T1A and was mainly distributed in the nuclei.By contrast,pancreatic triglyceride lipase expressed at a high level in T2A and was distributed diffusedly in the cytoplasm,nuclei and processes.Conclusion T1A and T2A showed different expression levels of pancreatic triglyceride lipase. Pancreatic triglyceride lipase expressed at a high level in T2A,which indicated that T2A may play an important role in the metabolism of lipids in the central nervous system.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第2期193-196,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30772242)
江苏省自然科学基金(BK1999084)
江苏省高校自然科学研究计划(05KJB310121)
江苏省分子神经生物学重点实验室项目(KJS02050)资助
