摘要
探索利用高效液相色谱法快速测定啤酒中亮蓝的方法。样品经简单处理,在627nm波长下,以甲醇∶乙酸铵溶液(0.02mol/L)为45∶55为流动相,进行高效液相色谱分析,以保留时间定性,与标准比较定量。结果表明,其回收率为94.0%～98.3%,最低检出浓度为0.01mg/kg;该方法简便、快速、灵敏度高、准确可靠。
Thempid measurement of brilliant blue content in bitter gourd beer by HPLC was investigated and the relative procedures were as follows： simple treatment of the samples, and then detected under 627 nm wavelength with moile phase as methyl alcohol ： acetic acid ammonium （0.02 mol/L）=45：55. The results showed that the recovery rate was between 94.0 %-98.3 % and the minimum detection limit was 0.01 mg/kg. Such method was simple and convenient and reliable with high sensitivity.
出处
《酿酒科技》
北大核心
2008年第4期112-113,共2页
Liquor-Making Science & Technology
