摘要
目的探讨HtrAl丝氨酸蛋白酶与妊娠期高血压疾病的关系。方法采用酶联免疫吸附试验法检测70例妊娠期高血压疾病患者（研究组）和30例正常妊娠孕妇（对照组）不同孕周外周血中HtrAl丝氨酸蛋白酶、转化生长因子β1水平。结果HtrAl丝氨酸蛋白酶在研究组和对照组血清中有表达，并随妊娠周数的增加HtrAl在血清中的表达水平逐渐降低，有两个低谷，且重度子痫前期组与对照纽相比HtrAl在孕28—32周时已明显下降，有显著性差异（X^2=8．00，P〈0．05）。重度子痫前期组孕晚期HtrAl、转化生长因子B1两因子的表达水平与正常组相比有显著差异（X^2分别为5．00、5．03，均P〈0．05），HtrAl显著降低，转化生长因子β1显著升高。结论HtrAl在妊娠期高血压疾病发病中可能有重要作用，参与胎盘浅着床，有望成为预测妊娠期高血压疾病发生发展的一项指标。
Objective To study relationship between high temperature factorA1 （HtrA1） serine protease and hypertensive disorder complicating pregnancy. Methods The levels of HtrA1 serine protease and transforming growth factor-betal （TGF-β1） in peripheral blood of 70 patients with hypertensive disorder complicating pregnancy （ as study group, including 24 cases of gestational hypertehsion, 20 cases of mild pre-eclampsia and 26 cases of severe pre-eclampsia） and 30 normal pregnant women （ as the controls） at different gestational weeks were detected by using ELISA. Results HtrA1 serine protease was expressed in serum of women both in the study group and the control group, and its expression level decreased gradually along with increase of gestational weeks with two ebb valleys. As compared with the control group, the expression level of HtrA1 began to decrease significantly in severe pre-eclampsia group from the 28th weeks to the 32th weeks and the difference was significant（X^2 = 8.00, P 〈 0.05 ）. In late pregnancy, in the expression levels of the two factors of HtrA1 and TGF-β1 in severe pre-eclampsia group, there were significant differences between the study group and the control group （for HtrA1 ： X^2 = 5.00, P 〈0.05;for TGF-β1：X^2 =5.03, P〈0.05） with decreased expression level of HtrA1 and increased expression level of TGF-β1. Conclusion HtrA1 participates in shallow implantation of fertilized ovum in placenta and may plays an important role in development of hypertensive disorder complicating oregnancv. It is hooeful to be a diagnostic index of hvoertensive disorder comolicating oregnancy.
出处
《中国妇幼健康研究》
2008年第2期102-104,F0003,共4页
Chinese Journal of Woman and Child Health Research
关键词
HtrAl
转化生长因子Β1
妊娠高血压疾病
血清
high temperature requirement factor A1 （ HtrA1 ）
transforming growth factor-betal （ TGF-IM ）
hypertensive disorder complicating pregnancy
serum
