摘要
目的探讨不同压力持续性CO2气腹对人卵巢癌SKOV3细胞裸鼠皮下移植瘤生长和转移的影响。方法将体外培养的人卵巢癌细胞株SKOV3分别暴露于不同压力(8、101、2 mmHg)和作用时间(13、h)的CO2环境中,对照组细胞放入CO2培养箱常规培养。收集各组细胞后接种于裸鼠腋下,建立有差异的裸鼠皮下移植瘤模型并绘制肿瘤生长曲线。接种6周后脱臼处死全部裸鼠,测量移植瘤的体积和重量,采用免疫组织化学法检测各组中增殖细胞核抗原(PCNA)和nm23-H1蛋白的表达。结果接种7 d后各组均明显成瘤,各CO2气体处理组的皮下移植瘤生长速度均快于对照组,其皮下移植瘤体积和重量也高于对照组(P<0.01)。各CO2处理组中肿瘤细胞PCNA蛋白表达阳性率均高于对照组(P<0.01),各组间nm23-H1蛋白表达阳性率差异无统计学意义(P>0.05)。结论CO2气体本身对人卵巢癌SKOV3细胞裸鼠皮下移植瘤的生长有明显的促进作用,对转移影响不大。
Objective To evaluate the effects of CO2 pneumoperitoneum on the growth and metastasis of the human ovarian carcinoma cell line SKOV3 in animal models. Methods First, the SKOV3 ovarian cancer cells were exposed to CO2-insufflation at different carbon dioxide pressures （8 mmHg, 10 mmHg and 12 mmHg） and effect times （ 1 h, 3 h）. Then, each mouse was inoculated with 0.2mL cell suspension （including 6.0×10^6 tumor cells）. The growth status of the tumor was regularly observed, and the growth curve was recorded. 6 weeks later, all mice were executed by luxation. The mice were immediately anatomized and the volume and weight of tumors were determined. Immunohistochemistry was used to determine the expression of PCNA protein and nm23-H1 protein of these cells. Results 7 days after hypodermic inoculation, tumor growth occurred in all the groups. The tumor growth was reinforced in the experimental groups compared with those of the control group （ P 〈 0.01 ）. The expression of PCNA protein was higher in experimental groups than that in the control group （P 〈 0.01 ）. But there was no significant difference between the groups in the expression of nm23-H1 protein（ P 〉 0.05）. Conclusion Carbon dioxide has auxoaction on the growth of the human ovarian carcinoma cell line SKOV3 xeno-transplanted into nude mice, but has no effects on tumor metastasis.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第3期254-257,共4页
Journal of Shandong University:Health Sciences
关键词
卵巢肿瘤
二氧化碳
小鼠
裸
移植瘤
Ovarian neoplasma
Carbon dioxide
Mice, nude
Xenograft tumor
