摘要
利用DAS-ELISA对荷兰进境的唐菖蒲培育叶片进行南芥菜花叶病毒检疫,再对血清呈阳性的种球进行培育,以长出的叶片作为检测材料,采用RT-PCR方法进行南芥菜花叶病毒的检测,并对PCR产物进行克隆与测序。结果表明,从唐菖蒲种球上发现了我国进境植物检疫二类危险性有害生物南芥菜花叶病毒(Arabismosaic virus,ArMV)。
With the methods of DAS-ELISA,Arabis mosaic virus（ArMV） was detected from Gladiolus hybridus bulb imported from Netherlands.The reverse transcription polymerase chain reaction（RT-PCR） was used to detect the virus and in the vercification of the plant,which was confirmed to carry the virus by DAS-ELISA.ArMV had been found from G.hybridus.The PCR product was cloned into the pMD-18T vector.Sequence determination confirmed that this sequence was a partical of ArMV coat protein gene.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2008年第1期46-48,共3页
Journal of Huazhong Agricultural University
基金
云南省检验检疫科技攻关项目(CIQ007-02)资助
