摘要
目的:建立云南民间常用药大叶紫珠中桦木酸的含量测定方法。方法:采用Kromasil-C18色谱柱(4.6mm×200mm,5μm),以乙腈-0.1%磷酸溶液(63∶37)为流动相,205nm为检测波长。结果:大叶紫珠中桦木酸与其他成分能得到很好的分离,考察3个不同来源的大叶紫珠叶中桦木酸的含量,不同来源桦木酸含量差异较大,其中云南大叶紫珠叶中桦木酸含量最高。测定线性范围为0.0166～0.332mg·mL-1,r=0.9998,平均回收率为98.5%。结论:该法便捷、灵敏、准确,重复性好,可以控制该药材的质量。
Objective： To establish an RP-HPLC method for determination of betulinic acid in Callicarpa maerophylla, a commordy used herbal in Yunnan. Method： A Kromasil-C18 column（4.6 mm ×200 mm, 5 μm）and a mobile phase consisted of aeetonitrile-0.1% phosphoric acid （63：37 ） were used. The flow rate was 1.0 mL · min^-1 and the UV detector wavelength was 205 nm. Result： Betulinic acid was well separated from other compounds in C. macrophylla. The content of betulinic acid in C. macrophylla from different origins showed apparent differences, the content of betulinic acid in C. macrophylla from Yunnan was the highest. The calibration curve was linear in the range of 0.016 6-0.332 mg · mL^-1 of betulinic acid with correlation coefficient 0.999 8. The average recovery of betulinic acid was 98.5%. Conclusion： The method is simple, accurate and reproducible, and is suitable for the quality control of C. macrophylla.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第7期753-755,共3页
China Journal of Chinese Materia Medica
