摘要
目的:探讨整合素连接激酶(integirn-linked kinase,ILK)反义寡核苷酸(antisense oligonucleotide,ASODN)对卵巢癌HO-8910细胞株中ILK基因表达的抑制作用。方法:将ILK反义寡核苷酸(ILK-ASODN)导入卵巢癌细胞株,阻断ILK的表达。转染后72h,用RT-PCR和Western-bllot法检测各组卵巢癌细胞ILKmRNA和蛋白表达量的变化。结果:ILK-ASODN处理后,各组卵巢癌细胞mRNA表达量明显下降,与两对照组比较差异有统计学意义(P<0.05),且mRNA表达量随转染浓度的增高而减低;各组蛋白表达量均显著下降,各组与两对照组比较差异有极显著意义(P<0.01),且蛋白表达量随转染浓度增高而减低。结论:ILK-ASODN导入卵巢癌细胞株后可以明显抑制卵巢癌HO-8910细胞株中ILK基因的mRNA表达和蛋白表达。
Objective： To explore the effect of integirn - linked kinase - antisense oligonucletide （ ILK - ASODN） on inhibiting ILK gene expression in human ovarian cancer cell lines （HO -8910）. Methods： We used lipofectamine 2000 to transfect ILK - SODN and ILK - ASODN into HO - 8910 to block ILK expression. The experiment had six groups, blank control （A）, lipofectamine 2000 control （B）, ILK-SODN 100nmol/L （C）, and three ILK - ASODN groups of 60nmol/L （D） , 80nmol/L （E） , and 100nmol/L （F）. 72h after transfection, we measured the expression levels of ILK mRNA by RT - PCR and ILK protein by Western - blotting. Results： After transfection of ILK- ASODN, the expression levels of mRNA decreased significantly in the experimental groups; group D showed significance （P〈0.05） and group E and F showed extremely significance （P 〈0.01） in comparison with the two control groups ; and the expression levels of mRNA decreased as the transfection levels increased. After ILK - SODN transfection, the expression levels of mRNA did not change significantly in group C （ P 〉 0.05 ）. After transfection of ILK - ASODN, the expression levels of protein decreased significantly in group D, E, F （ P 〈 0.01 ） in comparison with the two control groups ; the expression levels of protein decreased as the transfection levels increased. After ILK - SODN transfection, the expression levels of protein decreased significantly in group C （ P 〈 0.05 ）. Conclusion： Transfection of ILK - ASODN into human ovarian cancer line inhibited ILK gene and protein expression in HO - 8910.
出处
《现代肿瘤医学》
CAS
2008年第4期514-517,共4页
Journal of Modern Oncology
基金
黑龙江省卫生厅资助项目(2006-287)
哈尔滨医科大学青年科学基金资助项目(060009)
哈尔滨医科大学研究生创新基金资助项目
关键词
整合素连接激酶
反义脱氧寡核苷酸
卵巢癌细胞株
蛋白表达
integrin -linked kinase
antisense deoxy -oligonucleotide
human ovarian cancer line
protein expres-sion
